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Phospho-PKC alpha (T638) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112020-100µl
$279.00Price
 
  
  
 
    
Fig1: Western blot analysis of Phospho-PKC alpha(T638) on different lysates using anti-Phospho-PKC alpha(T638) antibody at 1/1,000 dilution.
 
Positive control: 
 
  Lane 1: Hela 
 
  Lane 2: 293
 
Fig2: ICC staining Phospho-PKC alpha(T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
 
Fig3: ICC staining Phospho-PKC alpha(T638) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
 		 
  
 
 

 Bon Opus Cat. #BA112020
Size
100 µl

  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat

  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Thr638 of human PKC alpha.

  • Application Summary

    WB, ICC/IF, IHC, IP

  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.

  • ALTnames

    Protein kinase C alpha type

  • Background

    Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.(ET1702-17)