Phospho-p95/NBS1 (S343) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111476-100µl
$279.00Price

Fig1: ICC staining Phospho-p95/NBS1(S343) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-p95/NBS1(S343) antibody. Counter stained with hematoxylin.

 

Bon Opus Cat. #BA111476
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Ser343 of human p95/NBS1.
  • Application Summary

    WB, ICC/IF, IHC, IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1
  • Background

    Nijmegen breakage syndrome (NBS) is characterized by extreme radiation sensitivity, chromosomal instability and cancer. These phenotypes are similar to those of ataxia telangiectasia mutated (ATM) disease, where there is a deficiency in a protein kinase that is activated by DNA damage, indicating that the NBS1 (Nibrin) and ATM proteins may participate in common pathways. Nibrin is specifically phosphorylated in response to gamma-radiation, ultraviolet light and exposure to hydroxyurea. The phosphorylation of Nibrin requires catalytically active ATM. ATM physically interacts with and phosphorylates Nibrin on Serine 343 both in vitro and in vivo. Serine 343 is phosphorylated in vitro by ATM and the modification of this residue in vivo is essential for the cellular response to DNA damage. This response includes S-phase checkpoint activation, formation of the NBS1/Mrel1/Rad50 nuclear foci and rescue of hypersensitivity to ionizing radiation.(ET1607-5)

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