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Phospho-MEK1 (S218/S222) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111576-100µl
$279.00Price

Fig1: Western blot analysis of Phospho-MEK1(S218/S222) on different lysates using anti-Phospho-MEK1(S218/S222) antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: A431 

  Lane 2: Hela 

  Lane 3: 293T

Fig2: ICC staining Phospho-MEK1(S218/S222) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Phospho-MEK1(S218/S222) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111576
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Ser218 and 222 of human MEK1.
  • Application Summary

    WB, ICC/IF, IHC, IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1
  • Background

    Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.(ET1609-50)