Phospho-Histone H3 (S10) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111307-100µl
$279.00Price

Fig1: Western blot analysis of Phospho-Histone H3(S10) on Hela cell lysates using anti-Phospho-Histone H3(S10) antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: Untreated HeLa cell lysate 

  Lane 2: HeLa cell lysate treated with calyculin A 

  Lane 3: HeLa cell lysate treated with calyculin A and alkaline phosphatase

Fig2: ICC staining Phospho-Histone H3(S10) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Phospho-Histone H3(S10) in Ags cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111307
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Ser10 of human Histone H3.
  • Application Summary

    WB, ICC/IF, IHC, IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Histone H3.3
  • Background

    In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.(ET1601-30)

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