Phospho-Histone H2A.X (S139) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Phospho-Histone H2A.X(S139) on HepG2 cell lysates using anti-Phospho-Histone H2A.X(S139) antibody at 1/1,000 dilution.
Lane 1: HepG2 cell lysate–treated with etoposide
Lane 3: HepG2 cell lysate–untreated
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Phospho-Histone H2A.X(S139) antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Ser139 of human Histone H2A.X.
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesHistone H2AX, Histone H2A.X
BackgroundHistone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. The H2AFX gene is located in close proximity to the Porphobilinogen deaminase (PBG-D) gene in both mouse and human, and maps to chromosome 9 and 11q23, respectively. H2A.X differs from the other members of the H2A family by the presence of a highly conserved C-terminal motif. It is rapidly phosphorylated in response to ionizing radiation and plays an important role in the recognition and repair of DNA double stranded breaks. The phosphorylated form of H2A.X, designated p-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA reaction that replaces one immunoglobulin heavy chain constant region gene with another. The phosphorylated p-H2A.X is also thought to initiate subsequent repair factors, including Rad50, Rad51 and BRCA1.(ET1602-2)