Phospho-H1.3+H1.4 Recombinant Rabbit monoclonal Ab IgG
Fig1: Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on CRC cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution.
Lane 1: Untreated CRC whole cell lysates Lane 2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates
Fig2: ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Thr17 of human H1.4.
Application SummaryWB, ICC/IF, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesHistone H1.3, Histone H1c, Histone H1s-2, Histone H1.4, Histone H1b, Histone H1s-4
BackgroundEukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.(ET1602-11)