Phospho-FOXO3a (S253) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Phospho-FOXO3a(S253) on different lysates using anti-Phospho-FOXO3a(S253) antibody at 1/1,000 dilution.
Lane 1: Hela
Lane 2: PC12
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-FOXO3a(S253) antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Ser253 of human FOXO3a.
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesForkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1
BackgroundFKHRL1 (for forkhead in rhabdomyosarcoma) is a member of the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at threonine-32 and serine-253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephospho-rylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.(ET1609-49)