Phospho-eIF4E (S209) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111534-100µl
$279.00Price

Fig1: Western blot analysis of Phospho-eIF4E(S209) on different lysates using anti-Phospho-eIF4E(S209) antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: Mouse spleen 

  Lane 2: Rat spleen

Fig2: ICC staining Phospho-eIF4E(S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Phospho-eIF4E(S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111534
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Ser209 of human eIF4E.
  • Application Summary

    WB, ICC, IHC, IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
  • Background

    The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.(ET1608-66)

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