Phospho-eIF-2a (S51) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Phospho-eIF-2a(S51) on different lysates using anti-Phospho-eIF-2a(S51) antibody at 1/1,000 dilution.
Lane 1: Hela
Lane 2: HUVEC
Lane 3: PC12
Fig2: ICC staining Phospho-eIF-2a(S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a(S51) antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Ser51 of human eIF-2a.
Application SummaryWB, ICC/IF, IHC, IP, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesEukaryotic translation initiation factor 2A, 65 kDa eukaryotic translation initiation factor 2A
BackgroundPhosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.(ET1603-14)