Phospho-EGFR (Y1092) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111446-100µl

Fig1: Western blot analysis of Phospho-EGFR(Y1092) on different lysates using anti-Phospho-EGFR(Y1092) antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: A431 treated with EGF 

  Lane 2: Untreated A431

Fig2: ICC staining Phospho-EGFR(Y1092) in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-EGFR(Y1092) antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA111446
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse
  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Tyr1092 of human EGFR.
  • Application Summary

  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
  • Background

    Epidermal growth factor mediates its effects on cell growth through its inter-action with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.(ET1606-44)

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