PFKFB3 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112347-100µl
$279.00Price

Fig1: Western blot analysis of PFKFB3 on different cell lysates using anti-PFKFB3 antibody at 1/1,000 dilution.

Positive control:

Lane 1: Hela

Lane 2: PC-12

Lane 3: A431

Fig2: ICC staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining PFKFB3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112347
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, ICC, IHC, FC, IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme, Renal carcinoma antigen NY-REN-56, iPFK-2, 6-phosphofructo-2-kinase, Fructose-2,6-bisphosphatase
  • Background

    Among the enzymes playing role in glycolysis,four allosteric PFKFB enzymes 1–4 expressed by four independent PFKFB genes, catalyze the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate, using ATP as the energy source in the glycolysis pathway. Among these four allosteric enzymes, PFKFB3 enzyme retains the highest Kinase/Biphosphatase activity ratio and is expressed by PFKFB3 gene which has been demonstrated to be highly expressed in leukemic cells and in solid tumors. Moreover, mitogenic, hypoxic and inflammatory conditions have an inductive effect on the expression of PFKFB3. Hence upregulation of PFKFB genes specific to cancer cells compared to their normal counterparts (from the same patients) with more robust over-expression in breast and lung cancer make it a more appropriate target.(ET1705-66)

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