PDPK1 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of PDPK1 on human PDPK1 recombinant protein using anti-PDPK1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of PDPK1 on HEK293 (1) and PDPK1-hIgGFc transfected HEK293 (2) cell lysate using anti-PDPK1 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of PDPK1 on different cell lysate using anti-PDPK1 antibody at 1/1,000 dilution.
Positive control: Line1: MCF-7 Line2: Hela Line3: K562 Line4: U937 Line5: A549 Line6: NIH/3T3 Line7: Jurkat Line8: PC-12 Line9: Cos7
Host Species; Species ReactivityMouse; Human, Mouse, Monkey, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesRibosomal protein S6 kinase alpha-2, 90 kDa ribosomal protein S6 kinase 2, MAP kinase-activated protein kinase 1c, Ribosomal S6 kinase 3, pp90RSK3
BackgroundPDPK1 (3-phosphoinositide dependent protein kinase 1), also known as PDK1, PDPK2, PDPK2P or PRO0461, is 556 amino acid ubiquitously expressed protein that localizes to the cell membrane, cytoplasm and nucleus. Acting as a master kinase, PDPK1 phosphorylates and activates a subgroup of the AGC family of protein kinases. PDPK1 is involved in mediating signal transduction for controlling proliferation, survival, and growth of developing pancreatic beta cells, regulating Ca2+ uptake and Ca2+-activated K+ channels of mast cells, regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 contains a PH domain, which play an essential role in homodimerization, localization and nuclear import of PDPK1, and a protein kinase domain. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3.(EM1706-41)