PDI Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
Lane 1: Hela
Lane 2: MCF-7
Lane 3: PANC
Lane 4: HepG2
Lane 5: HUVEC
Lane 6: NIH/3T3
Lane 7: L929
Lane 8: F9
Lane 9: Jurkat
Lane 10: A431
Lane 11: A549
Lane 12: Mouse liver
Lane 13: Mouse kidney
Lane 14: Mouse brain
Lane 15: Human kidney
Lane 16: Human brain
Lane 17: Human liver
Fig2: ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesProtein disulfide-isomerase A2,Pancreas-specific protein disulfide isomerase
BackgroundProtein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Another major function of PDI relates to its activity as a chaperone; i.e., it aids wrongly folded proteins to reach a correctly folded state without the aid of enzymatic disulfide shuffling. PDI has been found to be involved in the breaking of bonds on the HIV gp120 protein during HIV infection of CD4 positive cells, and is required for HIV infection of lymphocytes and monocytes.(ER40404)