p60 CAF1 Recombinant Rabbit monoclonal Antibody IgG

p60 CAF1 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112643-100µl
$279.00Price

Fig1: Western blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution.

 Positive control:

 Lane 1: SiHa 

      Lane 2: K562

 Lane 3: A431

 

Fig2: ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining p60 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112643
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein corresponding to the C-terminus of Human p60 CAF1.
  • Application Summary

    WB,ICC,IF,IP,IHC,FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7
  • Background

    Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.(ET7108-85)

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