NSE Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of NSE on different lysates using anti-NSE antibody at 1/1,000 dilution.
Lane 1: HepG2
Lane 2: Hela
Lane 3: 293
Fig2: ICC staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat, Zebrafish
Application SummaryWB, ICC, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesGamma-enolase, 2-phospho-D-glyceRate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase
BackgroundEnolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, α Enolase, β Enolase and γ Enolase. α Enolase expression has been detected on most tissues, whereas β Enolase is expressed predominantly in muscle tissue and γ Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.(ET1610-96)