nNOS Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on SHG-44(1) and mouse brain(2) lysates using anti-nNOS rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of SK-BR-3 cells using anti-nNOS rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of HepG2 cells using anti-nNOS rabbit polyclonal antibody.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNitric oxide synthase, brain,Constitutive NOS，NC-NOS，NOS type I，Neuronal NOS,Peptidyl-cysteine S-nitrosylase NOS1,bNOS
BackgroundNitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.(R1510-28)