NFkB p105/p50 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of NFkB p105/p50 on different lysates using anti-NFkB p105/p50 antibody at 1/1,000 dilution.
Lane 1: PC-12
Lane 2: NIH/3T3
Lane 3: Hela
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFkB p105/p50 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFkB p105/p50 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
BackgroundProteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF B (p50 and p65) and the Drosophila maternal morphagen, dorsal. These proteins share sequence homology over a region of 300 amino acids at their NH2-terminus, the region that contains their DNA binding and dimerization domains. The DNA binding activity of NF B is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described. These proteins, designated p105 and p100, are highly related but map on different chromosomes. The p105 (p110) precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated PdI, binds to p50 and regulates its activity.(ET1603-18)