NF-κB p65 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of NF-κB p65 on different lysates using anti-NF-κB p65 antibody at 1/1000 dilution.
Lane 1: Hela
Lane 2: A549
Lane 3: PC12
Lane 4: Mouse embryonic stem cell
Lane 5: NIH/3T3
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p65 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NF-κB p65 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenThis antibody is produced by immunizing rabbits with a synthetic Peptide (KLH-coupled) corresponding to N-terminal NF-κB p65.
Application SummaryWB, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTranscription factor p65,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
BackgroundNF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.(ER0815)