MICA + MICB Rabbit polyclonal Antibody IgG

SKU: BA111274-100µl

Fig1: Western blot analysis of MICA + MICB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Positive control:

Lane 1: U937

Lane 2: Mouse bone marrow

Fig2: ICC staining MICA + MICB in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MICA + MICB at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Fig3: Immunohistochemical analysis of paraffin-embedded rat skin tissue using anti-MICA + MICB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-95) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Bon Opus Cat. #BA111274
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Recombinant protein within Human MICA + MICB aa 180-310.
  • Application Summary

    WB, ICC, IHC
  • Purification; Formulation

    Protein affinity purified.; 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • Background

    MHC class I polypeptide-related sequence A/B (MICA/B) is a cell surface glycoprotein encoded by the MICA/B gene located within MHC locus. MICA and MICB are related to MHC class I and has similar domain structure, which is made up of external α1α2α3 domain, transmembrane segment and C-terminal cytoplasmic tail. MICA/B rather functions as a stress-induced ligand for NKG2D receptor. MICA is broadly recognized by NK cells, γδ T cells, and CD8+ αβ T cells which carry NKG2D receptor on their cell surface. As a result of NKG2D-MICA engagement, effector cytolytic responses of T cells and NK cells against epithelial tumor cells expressing MICA are initiated. MICA/B are not associated with β2-microglobulin nor binds peptides as conventional MHC class I molecules do. The heat shock stress pathway is involved in the regulation of MICA expression as transcription of MICA is regulated by promoter heat shock element.(ER1803-95)

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