MHC class I Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112050-100µl
$279.00Price

Fig1: Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: Raji 

  Lane 2: THP-1

Fig2: ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA112050
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, ICC/IF, IHC, FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen A*1, HLA class I histocompatibility antigen, B-7 alpha chain, MHC class I antigen B*7, HLA class I histocompatibility antigen, A-68 alpha chain, Aw-68, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A*68, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A*2, HLA class I histocompatibility antigen, A-11 alpha chain, MHC class I antigen A*11, HLA class I histocompatibility antigen, A-30 alpha chain, MHC class I antigen A*30, HLA class I histocompatibility antigen, A-26 alpha chain, MHC class I antigen A*26
  • Background

    Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.(ET1702-47)

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