MGMT Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111955-100µl

Fig1: Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1,000 dilution.

Positive control:   

Lane 1: MCF-7           

Lane 2: Hela

Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.

Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA111955
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, IHC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
  • Background

    MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.(ET1701-55)

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