MGMT Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.
Lane 1: Hela
Lane 2: MCF-7
Lane 3: Daudi
Lane 4: Raji
Lane 5: SK-BR-3
Lane 6: SKOV3
Lane 7: Human liver
Lane 8: Human kidney
Lane 9: Human thymus
Lane 10: Human placentae
Fig2: ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMethylated-DNA--protein-cysteine methyltransferase,6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase
BackgroundMGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.(ER40104)