MGMT Rabbit polyclonal Antibody IgG

SKU: BA110465-100µl
$279.00Price

Fig1: Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.

Positive control:   

Lane 1: Hela          

Lane 2: MCF-7 

Lane 3: Daudi         

Lane 4: Raji 

Lane 5: SK-BR-3       

Lane 6: SKOV3 

Lane 7: Human liver   

Lane 8: Human kidney 

Lane 9: Human thymus   

Lane 10: Human placentae

Fig2: ICC staining MGMT in A172 cells (green).  Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining MGMT in SK-BR-3 cells (green).  Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA110465
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, IHC, FC
  • Purification; Formulation

    Peptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Methylated-DNA--protein-cysteine methyltransferase,6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase
  • Background

    MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.(ER40104)

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