MCM5 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of MCM5 on different lysates using anti-MCM5 antibody at 1/500 dilution.
Lane 1: Mouse thymus tissue
Lane 2: SiHa
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse
ImmunogenRecombinant protein within C terminal human MCM5.
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesDNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46
BackgroundThe mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6.(ET7107-15)