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©2020 by Bon Opus Biosciences, LLC.

MARK3 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112689-100µl
$279.00Price

Fig1: Western blot analysis of MARK3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Positive control: 

Lane 1: rat brain tissue lysate

Lane 2: A431 cell lysate

Lane 3: 293 cell lysate

 

Fig2: Immunocytochemistry staining MARK3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Fig3: Immunocytochemistry staining MARK3 in SH-SY-5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Bon Opus Cat. #BA112689
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Recombinant protein within human Mark3 aa 600-750.
  • Application Summary

    WB, IP, ICC/IF, IHC, FC
  • Purification; Formulation

    Protein A purified.; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • Background

    MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 and is ubiquitously expressed in various human tissue and cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family and the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 and mouse chromosome 12 F1.(ET7109-39)