MARK2 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of MARK2 on MCF-7 (1) and SK-Br-3 (2) cell lysate using anti-MARK2 antibody at 1/2,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin.
Fig3: Flow cytometric analysis of MCF-7 cells with MARK2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenRecombinant protein near the C-terminus of human MARK2.
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesSerine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b
BackgroundMicrotubule affinity-regulating kinase 2 (MARK2), also known as EMK1 (ELKL motif kinase 1) or Par1b, is a 788 amino acid protein that is a member of the protein kinase superfamily, MARK subfamily. Highly expressed in heart, brain, skeletal muscle and pancreas, MARK2 is essential for the asymmetric development of membrane domains around polarized epithelial cells. Activation of MARK2 by phosphorylation on Thr 208 allows the protein to modulate the building of a columnar versus a hepatic epithelial cell. MARK2 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. MARK2 is expressed as 14 isoforms produced by alternative splicing events. Some of these isoforms may function in graft rejection.(ET7109-20)