MAPK14 Mouse monoclonal Antibody IgG2a
Fig1: Western blot analysis of MAPK14 on different cell lysates using anti-MAPK14 antibody at 1/500 dilution.
Lane 1: A431
Lane 2: HepG2
Lane 3: Hela
Lane 4: NIH/3T3
Lane 5: Jurkat
Lane 6: 293T
Lane 7: mouse placenta
Fig2: ICC staining of MAPK14 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse
Application SummaryWB, ICC, IHC
Purification; FormulationProG affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMitogen-activated protein kinase 14,Cytokine suppressive anti-inflammatory drug-binding protein,MAP kinase MXI2,MAX-interacting protein 2,Mitogen-activated protein kinase p38 alpha,Stress-activated protein kinase 2a
BackgroundMitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response.(EM40307)