Mad2L2 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, REV7 homolog
BackgroundCell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.(ET1706-38)