LRRK1 Mouse monoclonal Antibody IgG2b
Fig1: Western blot analysis of LRRK1 on A549 lysate using anti-LRRK1 antibody at 1/100 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse
Application SummaryWB, IHC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesLeucine-rich repeat serine/threonine-protein kinase 1
BackgroundLeucine-rich repeat serine/threonine-protein kinase 1 is an enzyme that in humans is encoded by the LRRK1 gene. Formation of LRRK1:EGF-R complexes result in LRRK cellular translocation: LRRK1 traffics together with EGF-R to endosomes upon stimulation of the receptor with EGF. LRRK1 is related to the familial Parkinsonism gene product Park8/LRRK2, and contains a Ras of complex proteins (ROC) GTPase domain and a MAPKKK-like kinase domain. LRRK1 participates in the intercellular trafficking of the epidermal growth factor (EGF) receptor. LRRK1 is activated by the sequential phosphorylation of 2 mitotic kinases, PLK1 and CDK1. Importantly, this PLK1-dependent activation of LRRK1 is required for the regulation of mitotic spindle orientation.LRRK1 regulates spindle orientation in a manner dependent on its kinase activity. Furthermore, the spindle misorientation caused by PLK1 inactivation is rescued by expression of the phospho-mimicking mutant LRRK1(S1790D). LRRK1 regulates spindle orientation downstream of PLK1.(M1701-14)