LGR5/GPR49 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of LGR5/GPR49 on SH-SY-5Y lysates using anti-LGR5/GPR49 antibody at 1/1,000 dilution.
Fig2: Flow cytometric analysis of SH-SY-5Y cells with LGR5/GPR49 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, IP, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesLeucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38
BackgroundG protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G-protein coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein.(ET1608-18)