KMT5A/SETD8/Pr-SET7 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution.
Lane 1: Mouse kidney
Lane 2: Mouse liver
Lane 3: 293T
Lane 4: 293
Fig2: Flow cytometric analysis of 293T cells with KMT5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Host Species; Species ReactivityRabbit; Human, Mouse
Application SummaryWB, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesN-lysine methyltransferase KMT5A, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, SET domain-containing protein 8
BackgroundProtein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. All monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions , benef playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contribute to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53 / TP53 at 'Lys-382', leading to repress p53 / TP53-target genes.(ET1705-59)