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Isocitrate dehydrogenase/IDH1 Mouse monoclonal Antibody IgG1

SKU: BA110408-100µl
$279.00Price

Fig1: Western blot analysis of IDH1 on different cell lysates using anti-IDH1 antibody at 1/1000 dilution.

Positive control:

Lane 1: Hela

Lane 2: HepG2

Lane 3: A431

Lane 4: MCF-7

Lane 5: A549

Lane 6: Jurkat

Lane 7: Human kidney

Lane 8: Human brain

Lane 9: Human liver

Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin.

Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IDH1 antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA110408
Size
  • Host Species; Species Reactivity

    Mouse; Human, Zebrafish
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, IHC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    IsocitRate dehydrogenase [NADP] cytoplasmic,Cytosolic NADP-isocitRate dehydrogenase,IDP,NADP(+)-specific ICDH,Oxalosuccinate decarboxylase
  • Background

    Isocitrate dehydrogenase (IDH) is an enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (α-ketoglutarate) and CO2. In humans, IDH exists in three isoforms: IDH3 catalyzes the third step of the citric acid cycle while converting NAD+ to NADH in the mitochondria. The isoforms IDH1 and IDH2 catalyze the same reaction outside the context of the citric acid cycle and use NADP+ as a cofactor instead of NAD+. They localize to the cytosol as well as the mitochondrion and peroxisome. Mutations in IDH1 are also implicated in cancer. Originally mutations in IDH1 were detected in an integrated genomic analysis of human glioblastoma multiforme. In addition to being mutated in diffuse gliomas, IDH1 has also been shown to harbor mutations in human acute myeloid leukemia (AML).(EM40705)