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IQGAP1 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112637-100µl
$279.00Price

Fig1: Western blot analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution.

 Positive control:

 Lane 1: HUVEC  

          Lane 2: A431

 Lane 3: Mouse placenta  

 

Fig2: ICC staining IQGAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining IQGAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112637
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse
  • Immunogen

    Recombinant protein corresponding to N-terminal human IQGAP1.
  • Application Summary

    WB,ICC,IF,IHC,FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Ras GTPase-activating-like protein IQGAP1, p195
  • Background

    IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.(ET7108-79)