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IKBKE Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112398-100µl
$279.00Price

Fig1: Western blot analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution.

Positive control:

 Lane 1: Hela

      Lane 2: Raji

 Lane 3: MCF-7

     Lane 4: Jurkat

 

Fig2: ICC staining IKBKE in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining IKBKE in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112398
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein
  • Application Summary

    WB,ICC,FC,IHC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase
  • Background

    The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKα and IKKβ. IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of IκBα on Ser 32 and Ser 36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system.(ET1706-20)