Ikaros Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Ikaros on human thymus tissue lysate using anti-Ikaros antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesDNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
BackgroundIkaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events.(ET7107-25)