Hsp90 alpha Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis on different cell lysates using anti-Hsp90 alpha Mouse mAb. Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: PC12
Lane 4: NIH/3T3
Fig2: Immunocytochemical staining of Hela cells using anti-Hsp90 alpha Mouse mAb.
Fig3: Immunocytochemical staining of SW480 cells using anti-Hsp90 alpha Mouse mAb.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesHeat shock protein HSP 90-alpha, Heat shock 86 kDa, Lipopolysaccharide-associated protein 2, Renal carcinoma antigen NY-REN-38
BackgroundThe heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.(M1603-3)