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©2019 by Bon Opus Biosciences, LLC.

HSP70 Rabbit polyclonal Antibody IgG

SKU: BA110363-100µl
$279.00Price

Fig1: Western blot analysis of HSP70 on different cell lysates using anti-HSP70 antibody at 1/500 dilution.

Positive control:   

Lane 1: A549           

Lane 2: MCF-7 

Lane 3: HCT116

Fig2: ICC staining HSP70 in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining HSP70 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA110363
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, IHC
  • Purification; Formulation

    Peptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Heat shock 70 kDa protein 1A,Heat shock 70 kDa protein 1
  • Background

    The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.(ER50802)