HSF1 Mouse monoclonal Antibody IgG2b
Fig1: Western blot analysis of HSF1 on human HSF1 recombinant protein using anti-HSF1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of HSF1 on HEK293 (1) and HSF1-hIgGFc transfected HEK293 (2) cell lysate using anti-HSF1 antibody at 1/1,000 dilution.
Fig3: ICC staining HSF1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, ICC, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesDeath-associated protein kinase 3, DAP-like kinase, MYPT1 kinase, Zipper-interacting protein kinase
BackgroundProkaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway(EM1706-45)