HPRT Recombinant Rabbit monoclonal Antibody IgG

HPRT Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112387-100µl

Fig1: Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution.

 Positive control:

 Lane 1: Hela         

  Lane 2: A431

 Lane 3: MCF-7        

  Lane 4: 293

 Lane 5: Rat kidney   

  Lane 6: Rat brain

 Lane 7: Mouse testis 

  Lane 8: Mouse colon


Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.

Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA112387
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant protein
  • Application Summary

  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Hypoxanthine-guanine phosphoribosyltransferase
  • Background

    HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.(ET1706-08)

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