HOXA9 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of HOXA9 on human HOXA9 recombinant protein using anti-HOXA9 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of HOXA9 on HEK293 (1) and HOXA9-hIgGFc transfected HEK293 (2) cell lysate using anti-HOXA9 antibody at 1/1,000 dilution.
Fig3: ICC staining HOXA9 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human, Mouse
Application SummaryWB, ICC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesBone morphogenetic protein 4, Bone morphogenetic protein 2B
BackgroundThe HOX homeobox genes encode proteins that play a role in embryonic development. The HOXA9 gene encodes a class I homeodomain protein, which is expressed in normal adult and fetal thymic tissue, and may play a role in regulating early differentiation of thymocytes. The HOXA9 homeodomain protein cooperatively binds consensus DNA sequences with Meis1 and Pbx1. In addition, the HOXA9 protein, along with the MEIS1 and Pbx 1 proteins, have been implicated in leukemic transformation in both mice and humans. Furthermore, overexpression of both HoxA9 and Meis1 in primary bone marrow cells in syngenic mice induced growth factor-dependent acute myeloid leukemia (AML). Chromosomal translocation of t(7;11)(p15;p15) has been demonstrated in patients with human AML and chronic myelogenous leukemia (CML), resulting in the fusion gene NUP98-HOXA9. Mice transplanted with bone marrow cells expressing NUP98-NOXA9 acquire a myeloproliferative disease (MPD) which ultimately degrades to AML.(EM1706-21)