Histone H3 (mono+di+methyl K79) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Histone H3(mono+di+tri methyl K79) on different lysates using anti-Histone H3(mono+di+tri methyl K79) antibody at 1/1,000 dilution.
Lane 1: Mouse testis
Lane 2: CRC
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3(mono+di+tri methyl K79) antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H3(mono+di+tri methyl K79) antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundEukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.(ET1602-41)