HIF1AN Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112611-100µl
$279.00Price

Fig1: Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.

Fig2: ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112611
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein within human HIF1AN aa 1-200.
  • Application Summary

    WB,ICC,IHC,FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Hypoxia-inducible factor 1-alpha inhibitor, Factor inhibiting HIF-1, Hypoxia-inducible factor asparagine hydroxylase
  • Background

    Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like β-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.(ET7108-50)

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