HDAC4 Recombinant Rabbit monoclonal Antibody IgG
SKU: BA111852-100µl
$279.00Price
Fig1: Western blot analysis of HDAC4 on different lysates using anti-HDAC4 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Fig2: ICC staining HDAC4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Fig3: ICC staining HDAC4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Size
100 µl
Host Species; Species Reactivity
Rabbit; HumanImmunogen
Recombinant proteinApplication Summary
WB, ICC/IFPurification; Formulation
ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.ALTnames
Histone deacetylase 4Background
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBPassociated factor), HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.(ET1612-51)