HDAC4 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of HDAC4 on human HDAC4 recombinant protein using anti-HDAC4 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of HDAC4 on HEK293 (1) and HDAC4-hIgGFc transfected HEK293 (2) cell lysate using anti-HDAC4 antibody at 1/1,000 dilution.
Fig3: ICC staining HDAC4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, ICC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesRas-related protein Rab-3A
BackgroundIn the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBP associated factor), HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.(EM1706-2)