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GNB2 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA113156-100µl
$279.00Price

Fig1: Western blot analysis of GNB2 on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GNB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GNB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Bon Opus Cat. #BA113156
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Synthetic Peptide within N terminal human GNB2.
  • Application Summary

    WB, IHC, FC
  • Purification; Formulation

    ProA purified.; 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
  • Background

    Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e., a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. Each subunit of the G protein complex is encoded by a member of one of three corresponding gene families (α, β, γ). In mammals, there are five different members of the β-subunit family. The β subunits of the G proteins are important regulators of G protein a subunits as well as of certain signal transduction receptors and effectors. In contrast to G β1-4, which are at least 83% homologous, G β5 is only 50% homologous to the other β subunits. Human G β5 is expressed at high levels in brain, pancreas, kidney, and heart.(ET7109-53)