GCLC Recombinant Rabbit monoclonal Antibody IgG

GCLC Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112237-100µl

Fig1:  Western blot analysis of GCLC on A431 cell using anti-GCLC antibody at 1/1,000 dilution.

Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GCLC antibody. Counter stained with hematoxylin.

Fig3: Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue using anti-GCLC antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA112237
  • Host Species; Species Reactivity

    Rabbit; Human, Rat
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, IHC, FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase
  • Background

    The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels.(ET1704-38)

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