GART Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution.
Lane 1: Hela
Lane 2: 293T
Lane 3: A431
Fig2: ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC/IF, IHC, IP
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTrifunctional purine biosynthetic protein adenosine-3, Phosphoribosylamine--glycine ligase, Glycinamide ribonucleotide synthetase, Phosphoribosylglycinamide synthetase, Phosphoribosylformylglycinamidine cyclo-ligase, AIR synthase, Phosphoribosyl-aminoimidazole synthetase, Phosphoribosylglycinamide formyltransferase, 5'-phosphoribosylglycinamide transformylase, GAR transformylase
BackgroundPurines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events.(ET1610-68)