GAPDH Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111309-100µl
$279.00Price

Fig1: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: NCCIT 

  Lane 2: PC12 

  Lane 3: F9

Fig2: ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111309
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat, Monkey, Chicken, Zebrafish
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, ICC/IF, IHC, IP, FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH
  • Background

    GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.(ET1601-4)

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