GAP43 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of GAP43 on mouse brain lysates using anti-GAP43 antibody at 1/1,000 dilution.
Fig2: ICC staining GAP43 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GAP43 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC/IF, IHC, IP, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNeuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46
BackgroundGAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein. Heterozygous GAP-43 knockout mice with GAP-43 levels reduced by one-half dipslay significant memory impairments in cued conditioning or on tests of nociceptive or auditory perception.(ET1610-94)