GAP43 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of GAP43 on different cell lysates using anti-GAP43 antibody at 1/1000 dilution.
Lane 1: Rat brain
Lane 2: Mouse brain
Lane 3: Mouse heart
Lane 4: Human skeletal muscle
Lane 5: N2A
Lane 6: A172
Lane 7: Human heart
Fig2: ICC staining GAP43 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GAP43 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNeuromodulin,Axonal membrane protein GAP-43,Calmodulin-binding protein P-57,Growth-associated protein 43
BackgroundGAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.(ER40201)