gamma tubulin Mouse monoclonal Antibody IgG2a

gamma tubulin Mouse monoclonal Antibody IgG2a

SKU: BA110856-100µl
$279.00Price

Fig1: Western blot analysis of gamma tubulin on different lysates using anti-gamma tubulin antibody at 1/2,000 dilution.

 Positive control:

 Lane 1: Jurkat     

   Lane 2: Hela   

 Lane 3: Human fetal brain tissue

Fig2: ICC staining gamma tubulin (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining gamma tubulin (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA110856
Size
  • Host Species; Species Reactivity

    Mouse; Human, Mouse, Rat
  • Immunogen

    Peptide
  • Application Summary

    WB, IHC, ICC
  • Purification; Formulation

    ProG affinity purified; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1
  • Background

    Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.(M1701-13)

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